Physiological Chemistry

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Chromatin organization and gene activity are responsive to developmental and environmental cues and much of gene regulation is highly cell-type specific. We developed and validated chromatin affinity purification from specific cell types by chromatin immunoprecipitation (CAST-ChIP), a broadly applicable biochemical procedure. CAST-ChIP powerfully identifies cell-type-specific as well as cell-type-invariant chromatin states, enabling the systematic dissection of chromatin structure and gene regulation within complex tissues such as the brain. more

Facilitates chromatin transcription (FACT) is a conserved histone chaperone that reorganizes nucleosomes and ensures chromatin integrity. Key to the broad functions of FACT is its recognition of histones H2A–H2B. In this report we reveal the crystal structure of the evolutionarily conserved FACT chaperone domain Spt16M in complex with the H2A–H2B heterodimer. We report the structure of the FACT heterodimerization domain that connects FACT to replicative polymerases. Our snapshot of the engagement of the chaperone with H2A–H2B and the structures of all globular FACT domains enable the high resolution analysis of the vital chaperoning functions of FACT. more