Micro-scale thermophoresis (MST)
Micro-scale thermophoresis (MST) is a powerful technique to quantify molecular interactions. During MST experiments the movement of molecules along a microscopic temperature gradient is measured. This motion is dependent on size, charge and hydration shell and changes when molecules interact. Molecular motion is monitored either via a fluorescent dye or protein (Monolith NT.115) or via intrinsic protein fluorescence (Monolith NT.LabelFree).
Micro-scale thermophoresis is used to measure the strength of molecular interactions. Typically, it is possible to analyse interactions with Kd values from the mM range to about 10 nM.
• 4 μl/capillary
• 250 μl of 0.5-1 μM protein with intrinsic protein fluorescence
• 50 μl of non-fluorescent binding partner at least 20x above Kd
• Monolith NT.LabelFree (Nanotemper)